結論

首先，表面張力測量證明牛精(jing)漿具有非常好(hao)的(de)表(biao)面(mian)活性(xing)，這可能與 BSP 蛋(dan)(dan)白(bai)(bai)。 二、朗繆爾薄(bo)膜(mo)法 先(xian)前(qian)被證明是相關的(de)脂質(zhi)(zhi)單層模型 公牛精(jing)子(zi)(zi)的(de)外細(xi)胞膜(mo) [35]，有助于表(biao)征(zheng) BSP 蛋(dan)(dan)白(bai)(bai)對脂質(zhi)(zhi)膜(mo)的(de)親(qin)和(he)力。 BSP 蛋(dan)(dan)白(bai)(bai)是 能夠到達被磷(lin)脂覆蓋的(de)牛精(jing)子(zi)(zi)細(xi)胞表(biao)面(mian)，這要歸功于它們(men)與磷(lin)脂酰膽堿和(he) 它們(men)自身(shen)的(de)表(biao)面(mian)活性(xing)。 我們(men)的(de)假設是，由于它們(men)自己的(de)表(biao)面(mian)，它們(men)首先(xian)穿透精(jing)子(zi)(zi)的(de)外葉 活動然后(hou)(hou)(hou)他(ta)們(men)留在(zai)那(nei)里因為(wei)與 磷(lin)脂酰膽堿。 最后(hou)(hou)(hou)，Langmuir 薄(bo)膜(mo)法也得到了 用于篩(shai)選(xuan)已知(zhi)的(de) BSP 蛋(dan)(dan)白(bai)(bai)螯合劑（如(ru) LDL 和(he)脂質(zhi)(zhi)體(ti)）的(de)作用。 脂質(zhi)(zhi)體(ti)被證明是 與 LDL 一樣(yang)有效地防止 BSP 蛋(dan)(dan)白(bai)(bai)插(cha)入 磷(lin)脂層。 發現 LDL 和(he) 脂質(zhi)(zhi)體(ti)：0.16–0.17 mg 磷(lin)脂酰膽堿/mg BSP。 我們(men)不 還知(zhi)道脂質(zhi)(zhi)體(ti)在(zai) 冷凍保存的(de)時(shi)間尺(chi)度有一些生物(wu)學后(hou)(hou)(hou)果(guo)。 需要進一步(bu)的(de)研究來分析(xi)每個的(de)表(biao)面(mian)特性(xing) BSP 蛋(dan)(dan)白(bai)(bai)及其(qi)對膜(mo)的(de)作用。 此外，可以測試朗繆爾薄(bo)膜(mo)以檢測 BSP 對膜(mo)的(de)親(qin)和(he)力隨溫度的(de)變化(hua) [20]。 最后(hou)(hou)(hou)，朗繆爾薄(bo)膜(mo)法是一種 用于鑒定新的(de)螯合劑的(de)強(qiang)大篩(shai)選(xuan)方(fang)法 BSP 蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)。 這種方(fang)法可以適(shi)用于其(qi)他(ta)動物(wu)物(wu)種。

致謝

作(zuo)者要感謝 IMV Technologies（法國） 其財政(zheng)支持，Gérard Chatagnon 的(de)量化(hua) 精漿中的(de)蛋白(bai)質，凝膠電泳的(de) Véronique Solé，英語校正(zheng)的(de) Maureen Collobert 和(he) Alain Sire 和(he) Patrice Papineau 的(de)概(gai)念和(he)實現 手(shou)套箱允(yun)許在受控的(de)情況(kuang)下使(shi)用 Langmuir 槽進行(xing)工作(zuo) 大氣層。

附(fu)錄 A. 補充(chong)數據

與本文(wen)相關的補充(chong)數據可以(yi)在(zai) 在(zai)線版本，見(jian) //dx.doi.org/10.1016/j.colsurfb.2015.11。 034.

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應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——摘要、簡介

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——材料與方法

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結果與討論

應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結論、致謝！