結論

在本(ben)研(yan)究(jiu)中(zhong)，朗繆(mou)爾單層(ceng)技(ji)術作為二維 方法適(shi)用(yong)于(yu)空氣-水(shui)/水(shui)界面 了解(jie)彼此之(zhi)間(jian)互(hu)動(dong)的(de)性(xing)質(zhi)(zhi)(zhi)和(he)方向 GO 和(he)脂(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)模型。 具有相(xiang)(xiang)同(tong) 18 碳烷基(ji)的(de)五種脂(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi) 鏈，但故意選擇(ze)不同(tong)的(de)頭組電(dian)荷 使可(ke)(ke)能的(de)相(xiang)(xiang)互(hu)作用(yong)合(he)理(li)化(hua)。 實驗(yan)結果(guo) 表明(ming)(ming)這(zhe)些脂(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)和(he) GO 之(zhi)間(jian)的(de)相(xiang)(xiang)互(hu)作用(yong)是明(ming)(ming)確的(de) 受靜(jing)電(dian)相(xiang)(xiang)互(hu)作用(yong)支配。 當這(zhe)些脂(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi) 散布在空氣-GO 分散界面，GO 可(ke)(ke)以結合(he) 或被吸附到單層(ceng)帶(dai)正電(dian)荷的(de)脂(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)中(zhong) DODAB 和(he) DSEPC，增(zeng)加(jia)平均(jun)分子面積(ji)。 然而，單層(ceng)帶(dai)中(zhong)性(xing)電(dian)荷的(de)頭基(ji) （磷(lin)(lin)酸(suan)膽堿）或帶(dai)負電(dian)荷的(de)頭部基(ji)團（磷(lin)(lin)酸(suan)和(he)羧基(ji)）不吸附 GO，因為沒有偏愛 靜(jing)電(dian)相(xiang)(xiang)互(hu)作用(yong)。 因為磷(lin)(lin)脂(zhi)(zhi)(zhi)(zhi)(zhi) 在生物系(xi)統中(zhong)帶(dai)負電(dian)或中(zhong)性(xing)電(dian)， GO 可(ke)(ke)能被細胞攝取到膜(mo)中(zhong) 是由于(yu) GO 和(he) 磷(lin)(lin)脂(zhi)(zhi)(zhi)(zhi)(zhi)，但通過(guo)膜(mo)的(de)生物活(huo)性(xing)。

當 GO 被注入到(dao) DODAB 和 DSEPC 帶(dai)正(zheng)電荷(he)單(dan)層(ceng)，不同 發現了表(biao)面壓(ya)(ya)力(li)的(de)(de)(de)(de)觀(guan)察結果。 GO 可以插(cha)入 單(dan)層(ceng) DODAB 以 20 mN/m 增加表(biao)面 壓(ya)(ya)力(li)。 然(ran)而，GO 不能(neng)(neng)擴(kuo)散到(dao)與 即使在(zai)低得多的(de)(de)(de)(de)表(biao)面壓(ya)(ya)力(li)下 DSEPC 單(dan)層(ceng) 可能(neng)(neng)是(shi)由于屏蔽了乙(yi)基磷基團。 GO 綁定(ding)到(dao) DODAB 時(shi)的(de)(de)(de)(de)定(ding)向(xiang)(xiang)模(mo)型和 提出 DSEPC 單(dan)層(ceng)來解釋不同的(de)(de)(de)(de) GO在(zai)空(kong)氣(qi)-水(shui)界(jie)面的(de)(de)(de)(de)吸(xi)附(fu)行為。 建(jian)議采用“邊緣向(xiang)(xiang)內”而不是(shi)“面向(xiang)(xiang)內”的(de)(de)(de)(de)方(fang)向(xiang)(xiang) 描述 GO 納米片(pian)插(cha)入時(shi)的(de)(de)(de)(de)方(fang)向(xiang)(xiang) DODAB 的(de)(de)(de)(de)單(dan)層(ceng)。

作者信息

通訊作者

*電子郵(you)件：rml@miami.edu (RML)。

筆記

作(zuo)者(zhe)聲明沒有競爭(zheng)性經濟(ji)利益。

致謝

這項(xiang)工作(zuo)得到了 2012 年橋梁基金資助 邁阿密大學(xue)。 

參考

(1) Dreyer, D. R.; Park, S.; Bielawski, C. W.; Ruoff, R. S. The Chemistry of Graphene Oxide. Chem. Soc. Rev. 2010, 39, 228?240.

(2) Geim, A. K.; Novoselov, K. S. The Rise of Graphene. Nat. Mater. 2007, 6, 183?191.

(3) Morales-Narvaez, E.; Merkoc ? i, A. Graphene Oxide as an Optical ? Biosensing Platform. Adv. Mater. 2012, 24, 3298?3308.

(4) Yang, X.; Wang, Y.; Huang, X.; Ma, Y.; Huang, Y.; Yang, R.; Duan, H.; Chen, Y. Multi-Functionalized Graphene Oxide Based Anticancer Drug-Carrier with Dual-Targeting Function and pHSensitivity. J. Mater. Chem. 2011, 21, 3448?3454.

(5) Nguyen, P.; Berry, V. Graphene Interfaced with Biological Cells: Opportunities and Challenges. J. Phys. Chem. Lett. 2012, 3, 1024? 1029.

(6) Li, S.; Aphale, A. N.; Macwan, I. G.; Patra, P. K.; Gonzalez, W. G.; Miksovska, J.; Leblanc, R. M. Graphene Oxide as a Quencher for Fluorescent Assay of Amino Acids, Peptides, and Proteins. ACS Appl. Mater. Interfaces 2012, 4, 7069?7075.

(7) Liu, Z.; Robinson, J. T.; Sun, X.; Dai, H. PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer Drugs. J. Am. Chem. Soc. 2008, 130, 10876?10877.

(8) Mu, Q.; Su, G.; Li, L.; Gilbertson, B. O.; Yu, L. H.; Zhang, Q.; Sun, Y. P.; Yan, B. Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets. ACS Appl. Mater. Interfaces 2012, 4, 2259?2266.

(9) Sharma, P.; Tuteja, S. K.; Bhalla, V.; Shekhawat, G.; Dravid, V. P.; Suri, C. R. Bio-Functionalized Graphene?Graphene Oxide Nanocomposite Based Electrochemical Immunosensing. Biosens. Bioelectron. 2013, 39, 99?105.

(10) Sun, X.; Liu, Z.; Welsher, K.; Robinson, J.; Goodwin, A.; Zaric, S.; Dai, H. Nano-Graphene Oxide for Cellular Imaging and Drug Delivery. Nano Res. 2008, 1, 203?212.

(11) Peng, C.; Hu, W.; Zhou, Y.; Fan, C.; Huang, Q. Intracellular Imaging with a Graphene-Based Fluorescent Probe. Small 2010, 6, 1686?1692.

(12) Wang, Y.; Zhen, S. J.; Zhang, Y.; Li, Y. F.; Huang, C. Z. Facile Fabrication of Metal Nanoparticle/Graphene Oxide Hybrids: A New Strategy To Directly Illuminate Graphene for Optical Imaging. J. Phys. Chem. C 2011, 115, 12815?12821.

(13) Wang, Y.; Li, Z.; Hu, D.; Lin, C.; Li, J.; Lin, Y. Aptamer/ Graphene Oxide Nanocomplex for in Situ Molecular Probing in Living Cells. J. Am. Chem. Soc. 2010, 132, 9274?9276.

(14) Zhang, M.; Yin, B.-C.; Wang, X.-F.; Ye, B.-C. Interaction of Peptides with Graphene oxide and Its Application for Real-Time Monitoring of Protease Activity. Chem. Commun. 2011, 47, 2399? 2401.

(15) Tian, B.; Wang, C.; Zhang, S.; Feng, L.; Liu, Z. Photothermally Enhanced Photodynamic Therapy Delivered by Nano-Graphene Oxide. ACS Nano 2011, 5, 7000?7009.

(16) Li, M.; Yang, X.; Ren, J.; Qu, K.; Qu, X. Using Graphene Oxide High Near-Infrared Absorbance for Photothermal Treatment of Alzheimer's Disease. Adv. Mater. 2012, 24, 1722?1728.

(17) Robinson, J. T.; Tabakman, S. M.; Liang, Y.; Wang, H.; Sanchez Casalongue, H.; Vinh, D.; Dai, H. Ultrasmall Reduced Graphene Oxide with High Near-Infrared Absorbance for Photothermal Therapy. J. Am. Chem. Soc. 2011, 133, 6825?6831.

(18) Spector, A. A.; Yorek, M. A. Membrane Lipid Composition and Cellular Function. J. Lipid Res. 1985, 26, 1015?35.

(19) Frost, R.; Jo? nsson, G. E.; Chakarov, D.; Svedhem, S.; Kasemo, B. Graphene Oxide and Lipid Membranes: Interactions and Nanocomposite Structures. Nano Lett. 2012, 12, 3356?3362.

(20) Chang, Y.; Yang, S.-T.; Liu, J.-H.; Dong, E.; Wang, Y.; Cao, A.; Liu, Y.; Wang, H. In Vitro Toxicity Evaluation of Graphene Oxide on A549 Cells. Toxicol. Lett. 2011, 200, 201?210.

(21) Liao, K.-H.; Lin, Y.-S.; Macosko, C. W.; Haynes, C. L. Cytotoxicity of Graphene Oxide and Graphene in Human Erythrocytes and Skin Fibroblasts. ACS Appl. Mater. Interfaces 2011, 3, 2607?2615.

 (22) Zhang, L. L.; Zhao, S.; Tian, X. N.; Zhao, X. S. Layered Graphene Oxide Nanostructures with Sandwiched Conducting Polymers as Supercapacitor Electrodes. Langmuir 2010, 26, 17624? 17628.

(23) Wang, Z.-M.; Wang, W.; Coombs, N.; Soheilnia, N.; Ozin, G. A. Graphene Oxide?Periodic Mesoporous Silica Sandwich Nanocomposites with Vertically Oriented Channels. ACS Nano 2010, 4, 7437? 7450.

 (24) Gao, Y.; Yip, H.-L.; Chen, K.-S.; O'Malley, K. M.; Acton, O.; Sun, Y.; Ting, G.; Chen, H.; Jen, A. K. Y. Surface Doping of Conjugated Polymers by Graphene Oxide and Its Application for Organic Electronic Devices. Adv. Mater. 2011, 23, 1903?1908.

(25) Engel, M. F. M.; Yigittop, H.; Elgersma, R. C.; Rijkers, D. T. S.; Liskamp, R. M. J.; de Kruijff, B.; Ho? ppener, J. W. M.; Killian, J. A. Islet Amyloid Polypeptide Inserts into Phospholipid Monolayers as Monomer. J. Mol. Biol. 2006, 356, 783?789.

(26) Evers, F.; Jeworrek, C.; Tiemeyer, S.; Weise, K.; Sellin, D.; Paulus, M.; Struth, B.; Tolan, M.; Winter, R. Elucidating the Mechanism of Lipid Membrane-Induced IAPP Fibrillogenesis and Its Inhibition by the Red Wine Compound Resveratrol: A Synchrotron X-ray Reflectivity Study. J. Am. Chem. Soc. 2009, 131, 9516?9521.

(27) Theumer, M. G.; Clop, P. D.; Rubinstein, H. R.; Perillo, M. A. Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes. J. Phys. Chem. B 2012, 116, 14216?14227.

(28) Si, Y.; Samulski, E. T. Synthesis of Water Soluble Graphene. Nano Lett. 2008, 8, 1679?1682.

(29) Constantine, C.; Elkind, B. J.; Leblanc, R. M. Encyclopedia of Surface and Colloid Science; Taylor & Francis Group: New York, 2006.

(30) Engelking, J.; Menzel, H. Adsorption of Anionic Polyelectrolytes to Dioctadecyldimethylammonium Bromide Monolayers. Eur. Phys. J. E 2001, 5, 87?96.

(31) Bao, Y.-Y.; Bi, L.-H.; Wu, L.-X.; Mal, S. S.; Kortz, U. Preparation and Characterization of Langmuir?Blodgett Films of Wheel-Shaped Cu-20 Tungstophosphate and DODA by Two Different Strategies. Langmuir 2009, 25, 13000?13006.

(32) MacDonald, R. C.; Gorbonos, A.; Momsen, M. M.; Brockman, H. L. Surface Properties of Dioleoyl-sn-glycerol-3-ethylphosphocholine, a Cationic Phosphatidylcholine Transfection Agent, Alone and in Combination with Lipids or DNA. Langmuir 2006, 22, 2770?2779.

(33) Zhang, H.; Peng, C.; Yang, J.; Lv, M.; Liu, R.; He, D.; Fan, C.; Huang, Q. Uniform Ultrasmall Graphene Oxide Nanosheets with Low Cytotoxicity and High Cellular Uptake. ACS Appl. Mater. Interfaces 2013, 5, 1761?1767.

(34) Dong, H.; Gao, W.; Yan, F.; Ji, H.; Ju, H. Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecules. Anal. Chem. 2010, 82, 5511?5517.

(35) Li, S.; Guo, J.; Patel, R. A.; Dadlani, A. L.; Leblanc, R. M. Interaction between Graphene Oxide and Pluronic F127 at the Air? Water Interface. Langmuir 2013, 29, 5742?5748.

(36) Gosvami, N. N.; Parsons, E.; Marcovich, C.; Berkowitz, M. L.; Hoogenboom, B. W.; Perkin, S. Resolving the Structure of a Model Hydrophobic Surface: DODAB Monolayers on Mica. RSC Adv. 2012, 2, 4181?4188.

石墨烯與磷脂之間的作用——摘要、介紹

石墨烯與磷脂之間的作用——實驗部分

石墨烯與磷脂之間的作用——結果和討論

石墨烯與磷脂之間的作用——結論、致謝！